用光譜寬度、光譜純度和熒光團兼容性,以實現激發和發射之間的很好區分。策略性的重復成像,輔以純凈、明亮、穩定的照明和高質量的光學玻璃,能夠實現高度多重化的結果,并克服了在可用可見波長內定義的歷史限制。參考文獻C Xia, J Fan, G Emanuel, J Hao, X Zhuang et al., Spatial transcriptome profiling by MERFISH reveals subcellular RNA compartmentalization and cell cycle-dependent gene expression Proc Natl Acad Sci ...
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